Prevention of recombinant human granulocyte colony stimulating factor(rhG-CSF;filgrastim) adsorption by addition of multi-vitamin mixture.
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چکیده
منابع مشابه
Production of Recombinant Human Granulocyte-Colony Stimulating Factor by Pichia pastoris
Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...
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Bakground: Granulocyte colony-stimulating factor (G-CSF) is a cytokine that stimulates hematopoiesis and induces proliferation and differentiation of granulocyte progenitor cells as well as production of bone marrow neutrophilic granulocyte colonies. Nowadays, human recombinant G-CSF(hr G-CSF)is used for the treatment of chemotherapy- and radiotherapy-induced neutropenia, and also in patients ...
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متن کاملproduction of recombinant human granulocyte-colony stimulating factor by pichia pastoris
human granulocyte-colony stimulating factor (hg-csf) cdna was expressed in the methylotrophic yeast pichia pastoris under the control of the alcohol oxidase (aox1) promoter. an expression vector for hg-csf secretion was constructed using vector ppic9. higher levels of hg-csf was obtained using a p. pastoris mut+ (methanol utilization fast) phenotype. the effects of environmental factors such as...
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A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of ?pL promoter under heat shock induction. Using the constructed vector,...
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ژورنال
عنوان ژورنال: Journal of the Japan Society of Blood Transfusion
سال: 1996
ISSN: 1883-8383,0546-1448
DOI: 10.3925/jjtc1958.42.162